http://hdl.handle.net/11422/114 Sun, 12 Apr 2026 03:29:23 GMT 2026-04-12T03:29:23Z http://hdl.handle.net/11422/27397 Title: Identification and structural-functional analysis of cyclin-dependent kinases of the cattle tick Rhipicephalus (Boophilus) microplus Author(s)/Inventor(s): Gomes, Helga Fernandes; Romeiro, Nelilma Correia; Braz, Glória Regina Cardoso; Oliveira, Eduardo Alves Gamosa de; Rodrigues, Camilla; Fonseca, Rodrigo Nunes da; Githaka, Naftaly; Isezaki, Masayoshi; Konnai, Satoru; Ohashi, Kazuhiko; Vaz Júnior, Itabajara da Silva; Logullo, Carlos; Moraes, Jorge Luiz da Cunha Abstract: Cyclin-dependent kinases (CDKs) are a family of serine/threonine kinases essential for cell cycle progression. Herein, we describe the participation of CDKs in the physiology of Rhipicephalus microplus, the southern cattle tick and an important disease vector. Firstly, amino acid sequences homologous with CDKs of other organisms were identified from a R. microplus transcriptome database in silico. The analysis of the deduced amino acid sequences of CDK1 and CDK10 from R. microplus showed that both have caspase-3/7 cleavage motifs despite their differences in motif position and length of encoded proteins. CDK1 has two motifs (DKRGD and SAKDA) located opposite to the ATP binding site while CDK10 has only one motif (SLLDN) for caspase 3–7 near the ATP binding site. Roscovitine (Rosco), a purine derivative that inhibits CDK/cyclin complexes by binding to the catalytic domain of the CDK molecule at the ATP binding site, which prevents the transfer of ATP's γphosphoryl group to the substrate. To determine the effect of Rosco on tick CDKs, BME26 cells derived from R. microplus embryo cells were utilized in vitro inhibition assays. Cell viability decreased in the Rosco-treated groups after 24 hours of incubation in a concentration-dependent manner and this was observed up to 48 hours following incubation. To our knowledge, this is the first report on characterization of a cell cycle protein in arachnids, and the sensitivity of BME26 tick cell line to Rosco treatment suggests that CDKs are potential targets for novel drug design to control tick infestation. Publisher: Public Library of Science Type: Artigo Fri, 11 Oct 2013 00:00:00 GMT http://hdl.handle.net/11422/27397 2013-10-11T00:00:00Z http://hdl.handle.net/11422/15325 Title: Repensando o ensino de anatomia humana para educação física baseado nas tendências educacionais do século XXI Author(s)/Inventor(s): Freitas, Emília Cristina Benevides de; Spiegel, Carolina Nascimento Abstract: The teaching of Human Anatomy still uses the traditional model, contentist, focused on memorization, making it uninteresting from the students' perspective. This work aims to present pedagogical innovations, built from the use of active learning methodologies and digital tools for Physical Education graduates. Research was carried out to ascertain educational trends for the 21st century. The following trends were incorporated: hybrid teaching, inverted classroom and gamification. For this, a Multimodal Hybrid Teaching Environment was created containing three online environments (Moodle platform, Facebook and Instagram) and two offline environments (theoretical classroom and practical laboratory). In addition, anatomical contents were approached in a systemic, multimodal, interdisciplinary manner and with a focus on professional practice. It is hoped that the proposal presented can contribute to the teaching and learning of Anatomy for undergraduate Physical Education students. Type: Artigo Thu, 29 Jul 2021 00:00:00 GMT http://hdl.handle.net/11422/15325 2021-07-29T00:00:00Z http://hdl.handle.net/11422/13014 Title: Notes on Mitobatinae III: a remarkable new Brazilian species of Mitobates Sund., 1833 (Opiliones, Laniatores, Gonyleptidae) Author(s)/Inventor(s): Kury, Adriano Brilhante Abstract: Mitobates inermis n. sp. is described from Rio de Janeiro state (Southeastern Brazil). Unlike most mitobatines the individuals of this species lack the paired spines in the area III of the mesotergum. Owing to this character, the species, following the Roewerian system, should be put in a new genus, nearest to Léptocnema C.L . Koch,1839, but in the phylogenetic approach adopted here it is placed nearest to Mitobates conspersus (Perty, 1833). Publisher: Museu Nacional Type: Artigo Mon, 30 Oct 1989 00:00:00 GMT http://hdl.handle.net/11422/13014 1989-10-30T00:00:00Z http://hdl.handle.net/11422/13009 Title: A new species of discocyrtoides (Opiliones: Gonyleptidae: Bourguyinae) Author(s)/Inventor(s): Kury, Adriano Brilhante Abstract: A new species of Discocyrtoides Mello-Leitao, from the southern part of the state of Espirito Santo (southeastern Brazil), Discocyrtoides pardus sp. n., is described. Discocyrtoides maculatus H. Soares is newly recorded from the same state (central part). Some brief comments are made on the systematic position of the Bourguyinae and their relationships with the Mitobatinae. Publisher: British Arachnological Society Type: Artigo Wed, 01 Mar 1989 00:00:00 GMT http://hdl.handle.net/11422/13009 1989-03-01T00:00:00Z