Nanopartículas de α-bisabolol em matriz de alginatoquitosana funcionalizadas com hialuronidase para aplicação no tratamento de hipercromia periorbital

Abstract

Pigmentary disorders are the third most common reason for looking for dermatological consultations. To minimize the effects of some of these disorders, one of the strategies adopted is the use of topical depigmenting agents. Therefore, since the objective of this work is to obtain nanomaterials with application in the treatment of periorbital hyperchromia, the active α-Bisabolol, which is a natural compound possessing antioxidant activity, was selected and suitable for this application. To guarantee the controlled release of the active and its protection, the nanoencapsulation of the active in matrix of alginato-chitosan, which are two biocompatible biopolymers, was carried out. In addition, to facilitate the penetration of the active in the skin, we also opted for the functionalization of the nanocapsules (NCs) with the enzyme hyaluronidase, which is able to make the extracellular matrix of the skin less dense through the degradation of hyaluronic acid, which today is one of the great challenges of the cosmetic industry. As a result of this research, NCs with ideal size for topical application and 98.5% of encapsulation efficiency were obtained. They remained stable during the 75 day trial and their antioxidant activity was confirmed by the DPPH method. Finally, they were functionalized with hyaluronidase extracted from bovine testicles, presenting 30.3% functionalization efficiency, 202.9 ± 6.7 nm in size and -15.93 ± 1.49 mV of zeta potential, in addition to the proven antioxidant activity, which makes them interesting for use in cosmeceuticals for the treatment of periorbital hyperchromia.